The ligand recogunition mechanism of Blasticidin S deaminase(BSD)
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چکیده
منابع مشابه
Acetylation of blasticidin S by its producing actinomycetes.
A blasticidin S-producing actinomycetes, Streptoverticillium sp. JCM 4673 possesses an enzyme activity which acetylates the drug in the presence of acetyl coenzyme A. The modified drug was biologically inactive when tested against protein synthesis in vivo and in vitro. Production of the enzyme which acetylates blasticidin S increases with formation of the antibiotic during cell growth.
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The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the final product BS. Incomplete conversion of LBS to BS at a variable efficiency rang...
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HWANG, B. K., and H. S. CHUNG. 1977. Acquired tolerance to Blasticidin-S in Pyricularia oryzae. Phytopathology 67: 421-424. Thirteen naturally-occurring isolates of Pyricularia oryzae transfers on fungicide-free media. Successive monoconidial varied in tolerance to Blasticidin-S [l-(1'-cytosinyl)-4-[L-3'cultures of Bu7 were genetically homogeneous for tolerance. amino-5-(l"-N-methylguanidino) v...
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BlsE, a predicted radical S-adenosyl-L-methionine (SAM) protein, was anaerobically purified and reconstituted in vitro to study its function in the blasticidin S biosynthetic pathway. The putative role of BlsE was elucidated based on bioinformatics analysis, genetic inactivation and biochemical characterization. Biochemical results showed that BlsE is a SAM-dependent radical enzyme that utilize...
متن کاملBlasticidin S inhibits translation by trapping deformed tRNA on the ribosome.
The antibiotic blasticidin S (BlaS) is a potent inhibitor of protein synthesis in bacteria and eukaryotes. We have determined a 3.4-Å crystal structure of BlaS bound to a 70S⋅tRNA ribosome complex and performed biochemical and single-molecule FRET experiments to determine the mechanism of action of the antibiotic. We find that BlaS enhances tRNA binding to the P site of the large ribosomal subu...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2001
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.41.s97_2